Bio-Rad, AHP3033, CENP-A (pSer18) antibody

Rabbit anti human CENP-A (pSer18) antibody recognizes centromere protein A when phosphorylated at serine 18. CENP-A is a histone H3 variant which replaces one or both canonical H3 histones in a subset of nucleosomes within centromeric chromatin and epigenetically determines the position of the centromere on each chromosome, and where kinetochore assembly and sister chromatid cohesion occurs during mitosis ( Fachinetti et al . 2013 ) ( Blower et al . 2002 ). CENP-A has been shown to interact directly with the inner kinetochore through CENP-C and CENP-N. This interaction allows microtubules to accurately segregate chromosomes during mitosis ( Kixomoeller et al . 2020 ) ( Yan et al . 2019 ). Phosphorylation of CENP-A on serine 18 is thought to play an important role in the segregation of chromosomes during mitosis ( Kixomoeller et al . 2020 ) ( Zhang et al . 2017 ). Rabbit anti human CENP-A (pSer18) antibody shows minimal cross-reactivity with CENP-A that has been phosphorylated at serine 16.

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.