Bio-Rad, AHP3099, RNA Pol II CTD (pSer5) antibody

Rabbit anti Human RNA pol II CTD (pSer5) antibody recognizes RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at serine 5°F the carboxyl-terminal repeat domain. RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA ( Kornburg, 1999 ) ( Sims et al . 2004 ). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle. It is thought that phosphorylation of the CTD of RNA pol II at serine 5 facilitates the formation of 3' ends of messages ( Phatnani and Greenleaf, 2006 ). Phosphorylation of serine 5°F the carboxyl-terminal repeat domain of RNA pol II facilitates the recruitment of the capping enzyme ( Egloff and Murphy, 2008 ). Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pSer5) based on sequence.

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.