Bio-Rad, AHP3100, RNA Pol II CTD (pThr4) antibody

Rabbit anti Human RNA pol II CTD (pThr4) antibody recognizes RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at threonine 4°F the carboxyl-terminal repeat domain. RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA ( Kornburg, 1999 ) ( Sims et al . 2004 ). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle ( Phatnani and Greenleaf, 2006 ). Phosphorylation of the CTD of RNA pol II at threonine 4 has been shown to facilitate histone mRNA 3' end processing ( Hsin et al . 2011 ). Phosphorylation of the carboxyl-terminal repeat domain of RNA polymerase II at threonine 4 occurs at the 3' end of most human genes and is necessary for transcription elongation ( Hintermair et al . 2012 ). Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pThr4) based on sequence.

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.