Bio-Rad, HCA395, Human IgG1 | AbD18705iq

Recombinant Human IgG1 LALA kappa allotype G1m3, clone AbD18705iq is a recombinant human antibody with a kappa light chain, and a heavy chain belonging to the G1m3 allotype, also designated G1m(f). It contains a LALA double mutation in its Fc region (mutation of leucine residues at positions 234 and 235 into alanine residues) which results in reducing its binding to FcγRI, II and III my more than a 100-fold, as well as reducing its ADCC function. This product is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a relevant control for scientists working on the R&D of therapeutic antibodies with this engineered Fc region. It is recommended that this reagent is used at the same concentration as the test reagent. The main allelic forms of IgG1 are G1m(f), G1m(f,a), and G1m(z,a). The G1m(f) (or G1m3) allele is only found in Caucasoid populations, whereas the G1m(f,a) allele is common in Mongoloids, but other variants, G1m(z,a,x) and G1m(z,a,v), have also been described; for further details on IgG1 allotypes see IgG subclasses and allotypes: from structure to effector functions , and IMGT Repertoire (IG and TR) Allotypes: Human ( Homo sapiens ) IGHC . The following publication reviews the serologically defined human IgG allotypes and considers the potential for allotype differences to contribute to or potentiate immunogenicity; Human immunoglobulin allotypes, possible implications for immunogenicity Our range of IgG1 controls also includes Recombinant Human IgG1 kappa allotype G1m17,1 , and the corresponding allotypes for IgG1 lambda. Another IgG1 control containing a N297Q mutation (mutation of the arginine residue at position 297 into a glutamine) preventing its binding to FcR1 is also available under product code HCA394 . View a summary of all recombinant fully human immunoglobulin isotype controls

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.