Bio-Rad, ICT9146, Pyroptosis FAM Caspase-1

Pyroptosis FAM Caspase-1 Kit utilizes the popular FLICA technology to detect caspase-1 activation. FLICA probes are cell permeant non-cytotoxic. This kits contain the caspase-1 inhibitor reagent YVAD-FMK, which has the preferred binding sequence for caspase-1, Tyr-Val-Ala-Asp (YVAD) ( Chapman, 1992 ). This preferred caspase-1 binding sequence is labeled with either FAM a green fluorescent dye and linked to a fluoromethyl ketone (FMK) reactive entity. Caspase-1 will not cleave the FLICA inhibitor probe; instead, it forms an irreversible covalent bond with the FMK group on the reagent and becomes inhibited from further enzymatic activity.

Storage Information
MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL. Store the unopened kit (and each unopened component) according to the storage instructions on each component label.
Store the Nigericin at -20°C. Once reconstituted the Nigericin stock should be used immediately or aliquoted and stored at -20°C for 12 months. Avoid repeated freezing and thawing.