Description
Use the Bio-Plex Pro cell signaling MAPK 9-plex panel, an all-in-one premixed multiplex kit, for detecting the following phosphorylated analytes from cell lysates derived from cell culture or tissue samples:
• ATF-2 (Thr 71 )
• ErK1/2 (Thr 202 / Tyr204 , Thr 185 /Tyr 187 )
• HSP27 (Ser 78 )
• JNK (Thr 183 /Tyr 185 )
• MEK1 (Ser 217 /Ser 221 )
• p38 MAPK (Thr 180 /Tyr 182 )
• p53 (Ser 15 )
• p90 RSK (Ser 380 )
• Stat3 (Ser 727 )
This assay panel measures the relative concentration of the phosphorylated protein.
Benefits
• All-in-one premixed kit
• Exceptional sensitivity (0.15 µg/well) and broad dynamic range
• Get started right away with the included assay quick guide
• Simplified assay processing with magnetic bead–based workflow to enable automation
Kit Components
• Coupled 6.5 µm magnetic beads
• Detection antibodies
• Cell lysate
• Cell lysis buffer
• Cell lysis factor QG
• Cell wash buffer
• Bead resuspension buffer
• Detection antibody diluent
• Wash buffer
• Streptavidin-PE
• 96-well flat bottom plate
• Sealing tape
Other Available Cell Signaling Assays
Browse our latest list of available phosphoprotein, total target, and housekeeping protein assays.
Other Assay Formats Available
Premixed all-in-one kits or custom Bio-Plex ® x-Plex™ assays.
Instrument Compatibility
Compatible with these readers and wash stations:
• Bio-Plex ® 200 or Luminex 200 system
• Bio-Plex ® 3D or Luminex FLEXMAP 3D system
• Bio-Plex ® MAGPIX™ or Luminex MAGPIX system
• Bio-Plex Pro™ wash stations
More Information
To learn more about performance characteristics or how to set up your cell signaling assays for optimal performance and sensitivity, refer to the documents listed below:
• Product information sheet
• Instruction manual
• Quick guide
