Description
Human Anti-Belimumab Antibody, clone AbD60131 is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug belimumab, and its biosimilar products. It does not recognize the drug target, the B-cell activating factor BAFF (CD257), nor belimumab in complex with BAFF. This antibody can be used to measure free belimumab levels in serum from patients. Clone AbD60131 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA0100 . It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 ( TZC002P ) for use in direct ELISA under product code TZA0100P . A pharmacokinetic (PK) bridging assay can be developed to measure free belimumab using clone AbD60131 in the purified Fab format TZA0100 as a capture antibody and the HRP conjugated bivalent Fab format TZA0100P for detection. Belimumab (trade name Benlysta) is a fully human monoclonal antibody of the IgG1/lambda isotype that binds to the B-cell activating factor BAFF to prevent its binding to B cells. Although part of the normal immune system, B cells are also responsible for the over-aggressive immune response observed in autoimmune diseases, such as systemic lupus erythematosus (SLE). Through its binding to BAFF, belimumab makes BAFF unavailable to B cells, which induces B cells apoptosis and therefore, a reduction in the overall number of circulating B cells. Therefore, belimumab acts as an immunosuppressant drug and is approved for the treatment of systemic lupus erythematosus. View a summary of all anti-belimumab antibodies
Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
