Description
Human Anti-Atezolizumab (Drug/Target Complex) Antibody, clone AbD40640 specifically recognizes the antibody drug atezolizumab when in complex with its target PD-L1 (programmed cell death ligand 1). It does not recognize free atezolizumab nor unbound PD-L1. It can be used to measure levels of atezolizumab in patient samples. This TrailBlazer Antibody is available in a purified monovalent Fab format tagged with a SpyTag2 at the C terminus of the Fab antibody heavy chain ( TZA013 ), enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. It is also available as a bivalent Fab format pre-coupled to the an HRP-conjugated BiSpyCatcher2 for direct detection of atezolizumab under product code TZA013P . View a summary of all SpyCatcher products Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free atezolizumab captured on immobilized PD-L1. Atezolizumab (brand name Tecentriq®) is a human monoclonal IgG1 antibody that binds directly to Programmed Death Ligand 1 (PD-L1, CD274) and provides a dual blockade of both the PD-1 and B7.1 receptors on T-cells and antigen presenting cells. It is one of the named “checkpoint inhibitor” drugs. PD-L1/PD-1 mediated inhibition of the immune response is thereby released, including reactivation of the anti-tumour immune response without induction of antibody-dependent cellular cytotoxicity. The drug is used to treat urothelial carcinoma, non-small cell lung cancer, triple-negative breast cancer, small cell lung cancer and hepatocellular carcinoma. It is used either on its own or in combination for cancers which are advanced or metastatic.
Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
