Bio-Rad, TZC026, Human IgG1-N297Q-FcSPYCATCHER3

Human IgG1-N297Q-FcSpyCatcher3 (hIgG1-N297Q-FcSpyCatcher3) contains a SpyCatcher3 ( Keeble et al. 2019 ) fused to the hinge region, CH2 and CH3°F human IgG1 ( Hentrich et al. 2020 ), resulting in a 77.2 kDa protein. It incorporates a N297Q mutation within its Fc region (mutation of the asparagine residue at position 297 into a glutamine) which can prevent binding to FcR1. Each SpyCatcher3 subunit can form a stable covalent isopeptide bond with a second protein that includes a SpyTag. The reaction occurs spontaneously on mixing, is rapid, irreversible, high-yielding and shows good specificity. The reaction is robust at pH 5 to 8, at temperatures from +4°C to +37°C, in various buffer conditions (Ca2 + /Mg2 + not needed) and in the presence of detergents. The reaction also occurs inside cells ( in vivo ). Since the speed of the coupling reaction is concentration dependent, it is recommended to use hIgG1-N297Q-FcCatcher3 undiluted. Human IgG1-N297Q-FcSpyCatcher3 is compatible with Bio-Rad's recombinant HuCAL antibodies with a SpyTag2 at the C-terminus of the heavy chain. Download FcSpyCatcher coupling protocol View all available Catchers

Storage Information
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.