Bio-Rad, TZC027, Human IgG1-LALA-FcSPYCATCHER3

Human IgG1-LALA-FcSpyCatcher3 (hIgG1-LALA-FcSpyCatcher3) contains a SpyCatcher3 ( Keeble et al. 2019 ) fused to the hinge region, CH2 and CH3°F human IgG1 ( Hentrich et al. 2020 ), resulting in a 77.0 kDa protein. It incorporates a LALA double mutation in its Fc region (mutation of leucine residues at position 234 and 235 into alanine residues) which results in reducing its binding to FcγRI, II and III by more than a 100-fold, as well as reducing its ADCC function. Each SpyCatcher3 subunit can form a stable covalent isopeptide bond with a second protein that includes a SpyTag. The reaction occurs spontaneously on mixing, is rapid, irreversible, high-yielding and shows good specificity. The reaction is robust at pH 5 to 8, at temperatures from +4°C to +37°C, in various buffer conditions (Ca2 + /Mg2 + not needed) and in the presence of detergents. The reaction also occurs inside cells ( in vivo ). Since the speed of the coupling reaction is concentration dependent, it is recommended to use hIgG1-LALA-FcCatcher3 undiluted. Human IgG1-LALA-FcSpyCatcher3 is compatible with Bio-Rad's recombinant HuCAL antibodies with a SpyTag2 at the C-terminus of the heavy chain. Download FcSpyCatcher coupling protocol View all available Catchers

Storage Information
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.