Bio-Rad, TZC036, Human IgM-FcSPYCATCHER3

Human IgM-FcSpyCatcher3 (hIgM-FcSpyC3) contains a SpyCatcher3 ( Keeble et al. 2019 ) fused to the hinge region, CH2 and CH3 domains of human IgM, resulting in a 102.4 kDa protein for each disulfide-bridge IgM monomer. Analysis of the hIgM-FcSpyCatcher3 (TZC036) shows that it spontanously forms pentameric and hexameric IgMs and that it does not contain any J chain. Each SpyCatcher3 subunit can form a stable covalent isopeptide bond with a second protein that includes a SpyTag. The reaction occurs spontaneously on mixing, is rapid, irreversible, high-yielding and shows good specificity. The reaction is robust at pH 5 to 8, at temperatures from +4°C to +37°C, in various buffer conditions (Ca2+/Mg2+ not needed) and in the presence of detergents. The reaction also occurs inside cells ( in vivo ). Since the speed of the coupling reaction is concentration dependent, it is recommended to use hIgM-FcCatcher3 undiluted. Human IgM-FcSpyCatcher3 has been successfully tested in ELISA, Western-Blotting and precipitation assays and is compatible with Bio-Rad's recombinant HuCAL antibodies with a SpyTag2 at the C-terminus of the heavy chain. hIgM-FcSpyCatcher3 coupling protocol View all available Catchers

Storage Information
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.