CST, 13296S, Mitochondrial Membrane Potential Assay Kit (II)

Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
The Mitochondrial Membrane Potential Assay Kit (II) is expected to detect the mitochondrial membrane potential in living cells cross all species. For the best result, a cell number titration is recommended when using a plate-reader and a 96-well plate.
Species Reactivity: All Species Expected
Background
Mitochondria are the main power house in cells and play important roles in processes such as steroid metabolism, calcium homeostasis, apoptosis and cellular proliferation. Mitochondrial membrane potential is a key indicator of its function and cell health (1,2). The dissipation of mitochondrial membrane potential is established as an early indicator for apoptosis (3). TMRE (tetramethylrhodamine, ethyl ester) is a cell membrane permeable cationic dye. In normal cells, TMRE accumulates in the mitochondria in response to their high membrane potential and negative charge. When excited at 550 nm, TMRE emits an orange-red fluorescence with a maximum at 575 nm (orange-red). Cells that have lost membrane potential or mitochondria activity cannot accumulate TMRE. Therefore, the fluorescence intensity of the orange-red emission can be used to measure mitochondria membrane potential and is an indicator for cell health (4).
Alternate Names
CCCP; TMRE