CST, 13859S, Cellular Glutathione Detection Assay Kit

Storage
Upon receipt, Tris Assay Buffer (#13865) should be removed from #13859 and stored at 4°C. And remaining components should be stored at -20ºC.
Protocol
Available protocols: Functional
Specificity / Sensitivity
The Cellular Glutathione Detection Assay Kit is expected to detect the reduced glutathione in cellular assays across all species.
Species Reactivity: All Species Expected
Background
The antioxidant glutathione is found in both reduced and oxidized states in cells. Reduced glutathione can play an important role in preventing cellular damage caused by reactive oxygen species, including free radicals and peroxides. Reduced glutathione (GSH) acts as an electron donor in the presence of free radicals and peroxides to become oxidized (GSSG). GSH also participates in redox signaling through the removal of the cellular second messenger H O (1,2). Diminished glutathione levels are observed during the aging process and in oxidative stress-related diseases. The depletion of GSH is necessary for the progression of apoptosis that is mediated by various signaling pathways (3,4). Intracellular GSH levels can be a very useful indicator for overall cell health, proliferation, and death (2).