CST, 16187S, Human GDNF Recombinant Protein

Storage
Human GDNF Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Source / Purification
Recombinant human GDNF was expressed in and is supplied in a lyophilized form.
Background
Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). This glycosylated, disulfide-bonded homodimer is a member of the TGF-β superfamily and plays an important role in neuronal survival (2). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor α (GFRα) proteins that signal through the Ret receptor tyrosine kinase (3,4). The effect that GDNF has on degenerating dopamine neurons makes it an important growth factor when studying Parkinson's disease (PD) (5). GDNF has also been identified as a therapeutic target for amyotrophic lateral sclerosis (ALS), is a suggested biomarker for Alzheimer's Disease (AD), and has been associated with cancer progression (6-9).