CST, 37969S, Bisindolylmaleimide I (GF-109203X)

Product Usage Information
Bisindolylmaleimide I (GF-109203X) is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 1 mg of powder in 0.24 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 2 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles .
Background
Bisindolylmaleimide I (BIS), also known as GF-109203X, is a potent inhibitor of PKC (1,2). the IC of BIS is 10-20 nM for PKCα/β/γ and 100-200 nM for PKCδ/ε isoforms. The IC for PKCζ is approximately 6 μM, indicating that BIS is a very weak inhibitor for this isoform. In cellular assays, the IC of BIS for PKC is 0.2-2 μM (1,3).
Alternate Names
2-[1-(3-Dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)maleimide; Bisindolylmaleimide I; Gö 6850