CST, 44695S, Human WISP2 Recombinant Protein

Storage
Human WISP2 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Source / Purification
Recombinant human WISP2 was expressed in and is supplied in a lyophilized form.
Background
WNT1 inducible signaling pathway protein 2 (WISP2 or CCN5) belongs to the CCN (Cyr61, Ctgf, NOV) family of proteins. It is a cysteine-rich secretory protein that associates with components of the extracellular matrix (1-3). WISP2 is expressed in endoderm, mesoderm, and ectoderm lineages, including primary osteoblasts, fibroblasts, ovaries, testes, and heart cells (3,4). This protein has been found to regulate cell proliferation, adhesion, signaling, wound healing, fibrosis, skeletal development, and metastasis (5). WISP2 promotes the adhesion of osteoblasts, inhibits osteocalcin production, and inhibits fibrinogen binding to integrin receptors (6). WISP2 is normally expressed in both embryonic and adult tissues (7,8).