CST, 46080S, UNC1999

Product Usage Information
UNC1999 is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 1 mg in 350.97 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 5 nM -5 μM for 2-48 hr.
Storage
Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Background
UNC1999 inhibits EZH2 and EZH1 histone-lysine N-methyltransferase activity through competitive inhibition of the cofactor S-Adenosyl-l-methionine (SAM). The UNC1999 inhibitor displays potent specificity relative to other histone methyltransferases. IC values for EZH2 and EZH1 are 2 nM and 45 nM, respectively (1). Research studies demonstrate that UNC1999 effectively blocks histone H3 lysine 27 (H3K27) methylation. UNC1999 induces anti-proliferation, cell differentiation, and apoptosis, while exhibiting low cellular toxicity (1,2).
Alternate Names
ezh1; ezh2; h3k27; histone; inhibitor; methylation; methyltransferase; sam cofactor