Description
Product Usage Information
Flow Cytometry (Live): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Live)
Specificity / Sensitivity
B7-H6 (E9Q7L) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total B7-H6 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro185 of human B7-H6 protein.
Background
B7-H6 (NCR3LG1) is a member of the B7 family of proteins that provide costimulatory signals for regulating T cell responses (1-3). B7-H6 is a ligand for the natural killer (NK)-cell-activating receptor NKp30, which triggers anti-tumor NK cell cytotoxicity and cytokine secretion (1). Both forms of B7-H6, cell surface B7-H6 and soluble B7-H6 (sB7-H6), are detected in primary tumors and the tumor microenvironment, but rarely in normal tissues (1-5). Cell surface B7-H6 associates with cell damage, and triggers innate immunity when it binds to NKp30 on NK cells (3,5). Soluble B7-H6 can decrease NKp30 expression on NK cells and induce NK cell dysfunction (4,5).
Alternate Names
B7 homolog 6; B7-H6; B7H6; DKFZp686I21167; DKFZp686O24166; hypothetical protein DKFZp686O24166; Natural cytotoxicity triggering receptor 3 ligand 1; natural killer cell cytotoxicity receptor 3 ligand 1; NCR3LG1; NR3L1; Putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167; YK047
