CST, 6301S, SignalSilence ® GSK-3 alpha/beta siRNA

Product Usage Information
CST recommends transfection with 50 nM SignalSilence ® GSK-3 alpha/beta siRNA 48 hours prior to cell lysis. See Protocol for transfection procedure.
Storage
GSK-3α/β siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Small Interfering RNA (siRNA) has been used to specifically silence GSK-3alpha and beta expression, causing an upregulation of beta-catenin protein levels (6).
Alternate Names
glycogen synthase kinase-3; GSK 3alpha; GSK 3beta; GSK-3alpha; GSK-3beta; gsk-3β; GSK3alpha; GSK3beta; gsk3α; gsk3β; RNAi; SignalSilence; siRNA