CST, 74054S, Necrosulfonamide

Product Usage Information
Necrosulfonamide is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.08 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Background
Necrosulfonamide is a potent, selective necroptosis inhibitor with an IC value of less than 0.2 µM. It inhibits mixed lineage kinase domain-like protein (MLKL), and blocks necrosis downstream of receptor-interacting serine-threonine kinase 3 (RIP3) activation. Necrosulfonamide has been shown to effectively prevent the MLKL-RIP1-RIP3 necrosome complex from interacting with downstream necrosis effectors (1).
Alternate Names
(2E)-N-[4-[[(3-Methoxy-2-pyrazinyl)amino]sulfonyl]phenyl]-3-(5-nitro-2-thienyl)-2- propenamide