CST, 79241S, PD 0325901

Product Usage Information
PD 0325901 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.03 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Background
PD 0325901 is a potent and selective non-ATP competitive MEK inhibitor. This small molecule is highly effective at inhibiting cell growth and proliferation in various cancer cells (1). The potency of PD 0325901 has been illustrated by the inhibition of ERK phosphorylation in mouse and human cell models with an IC of 0.33 nM and between 20-50 nM, respectively (1,2). PD 0325901 increases the self-renewal of mouse embryonic stem cells (mESCs) by enhancing the expression of pluripotency genes and acts as an antagonist for retinoic acid (RA) induced cell differentiation (3).
Alternate Names
N-[(2R)-2,3-Dihydroxypropoxy]-3,4-difluoro-2-[(2-fluoro-4-iodophenyl)amino]-benzamide; PD 0325901; PD-325901; PD0325901