CST, 84748S, PTMScan ® LysC Protease

Product Usage Information
For PTMScan ®  protocols, we recommend making a 5 mg/mL stock solution by resuspending 1 mg in 0.2 mL of 20 mM HEPES pH 8.0. Aliquot resuspended enzyme for single use. Store aliquots at -80°C. See the PTMScan ®  protocol for further details.
Storage
Lyophilized LysC has a shelf life of 12 months when stored at -20°C. Once reconstituted, the enzyme can be aliquoted and stored at -80°C for 6 months.
Specificity / Sensitivity
The activity of LysC is greater than or equal to 1 AU/mg.
Source / Purification
PTMScan LysC Protease was produced recombinantly in , purified and provided lyophilized in 1-5% Tris and 20-40% mannitol. Purity was accessed by SDS-PAGE. One amidase unit (AU) of the enzyme produces 1 μmol of p-nitroaniline per min at pH 9.5 at 30°C.
Background
LysC cleaves proteins on the carboxyl side of lysine residues. This makes it a useful tool in protein sequencing and proteomic applications. The enzyme was originally purified from the Gram-negative soil bacteria (1). It has been found to work optimally between 30-37°C and pH ranges from 9.0-9.5 but will undergo degradation at 50°C and higher. Activity is maintained in 4 M urea or 0.1% SDS solutions for up to 6 hr at 30°C. Inhibitors of the enzyme include PMSF, DFP, and TLCK.