Description
Storage
Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Single Cell Analysis - InTraSeq™ 3'
Specificity / Sensitivity
Bcl-xL (54H6) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3058) detects endogenous levels of total Bcl-xL protein. This antibody conjugate does not cross-react with other Bcl-2 family members.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL.
Background
Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5).
Alternate Names
Apoptosis regulator Bcl-X; B2CL1; Bcl-2-like protein 1; Bcl-X; bcl-xL; BCL-XL/S; bcl-xS; BCL2 like 1; Bcl2-L-1; BCL2-like 1; BCL2L; BCL2L1; BCLX; BCLXL; BCLXS; DKFZp781P2092; PPP1R52; protein phosphatase 1, regulatory subunit 52
