| Form | Column |
|---|---|
| Column format | Analytical |
| Phase | InertSustain C18 |
| Base Material | High Purity ES Silica Gel |
| Functional group | Octadecyl |
| Particle size | 5 µm |
| Length | 250 mm |
| Inner diameter | 4.6 mm |
| Mode | Reversed phase |
| Surface area | 350 m2/g |
| Pore size | 100 Å (10 nm) |
| Pore volume | 0.85 mL/g |
| End capping | Yes |
| Carbon loading | 14.0% |
| pH Range | 1-10 |
| Unit size | 1 pc. |
| Max Operating Pressure | 20 MPa(200 bar) |
| USP Code | L1 |
Answer:The column contains endcapped InertSustain C18 silica, 5 µm particles and 100 Å pores, in a 4.6 × 250 mm format.
Answer:The specified mobile-phase pH range is 1–10 and the maximum operating pressure is 20 MPa (200 bar).
Answer:Octadecyl-bonded silica provides hydrophobic retention in reversed-phase HPLC, with high inertness to reduce unwanted secondary interactions.
GL Sciences InertSustain C18 is a reversed-phase analytical HPLC column packed with endcapped octadecyl-bonded, high-purity ES silica. The 5 µm particles and 4.6 × 250 mm column format support established HPLC methods that require hydrophobic retention and high separation capacity. The stationary phase has a nominal 100 Å pore size, 350 m²/g surface area and 14% carbon loading. Its specified pH range is 1–10, with a maximum operating pressure of 20 MPa (200 bar) for this column format. Inert surface chemistry helps reduce interactions between basic analytes and residual silanol sites, supporting more consistent peak shapes. Use filtered HPLC-grade mobile phases, equilibrate the column to a stable baseline and pressure before injecting samples, and choose a flow rate compatible with the instrument and method. Avoid precipitation of buffer salts during solvent changes. This package contains one analytical column.
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