| Form | Column |
|---|---|
| Column format | Analytical |
| Phase | InertSustain C18 |
| Base Material | High Purity ES Silica Gel |
| Functional group | Octadecyl |
| Particle size | 5 µm |
| Length | 150 mm |
| Inner diameter | 3.9 mm |
| Mode | Reversed phase |
| Surface area | 350 m2/g |
| Pore size | 100 Å (10 nm) |
| Pore volume | 0.85 mL/g |
| End capping | Yes |
| Carbon loading | 14.0% |
| pH Range | 1-10 |
| Unit size | 1 pc. |
| Max Operating Pressure | 20 MPa(200 bar) |
| USP Code | L1 |
Answer:The column measures 3.9 × 150 mm and contains 5 µm reversed-phase C18 particles.
Answer:The packing has 100 Å pores and a maximum column pressure rating of 20 MPa, equivalent to 200 bar.
Answer:The stated mobile-phase pH range is 1 to 10, subject to the complete column operating instructions.
The GL Sciences InertSustain C18 HPLC column provides reversed-phase separation using a densely endcapped octadecyl stationary phase bonded to high-purity ES silica. Its 5 µm particles and 3.9 × 150 mm dimensions support analytical liquid chromatography methods that require conventional HPLC flow rates and manageable backpressure. The packing has nominal 100 Å pores and 14% carbon loading, giving hydrophobic retention for many organic compounds. Reduced activity at residual silica sites can improve peak symmetry for analytes susceptible to secondary interactions. The specified mobile-phase pH range is 1–10, and the maximum operating pressure is 20 MPa or 200 bar. Select buffer and organic modifier concentrations that maintain sample solubility and acceptable chromatographic resolution. Filter particulate-containing samples and condition the packed bed thoroughly after changing mobile phases. Use a compatible guard strategy when working with complex matrices. Monitor column pressure, peak width and retention time to assess performance throughout method development and routine testing.
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