| Form | Column |
|---|---|
| Column format | Analytical |
| Phase | InertSustain C18 |
| Base Material | High Purity ES Silica Gel |
| Functional group | Octadecyl |
| Particle size | 10 µm |
| Length | 300 mm |
| Inner diameter | 4.6 mm |
| Mode | Reversed phase |
| Surface area | 350 m2/g |
| Pore size | 100 Å (10 nm) |
| Pore volume | 0.85 mL/g |
| End capping | Yes |
| Carbon loading | 14.0% |
| pH Range | 1-10 |
| Unit size | 1 pc. |
| Max Operating Pressure | 20 MPa(200 bar) |
| USP Code | L1 |
Answer:The column is 4.6 mm in internal diameter and 300 mm long, with 10 µm packing particles.
Answer:No. Particle size and bed length alter efficiency, backpressure and method selectivity requirements.
Answer:The endcapped octadecyl silica phase is designed for reversed-phase liquid chromatography.
The GL Sciences InertSustain C18 column with 10 µm packing and a 300 mm bed length is a specialized analytical-format reversed-phase separation column. Its nominal 4.6 mm internal diameter and long packed bed distinguish it from the shorter 100 mm and 150 mm members of the same C18 family. The chemically bonded octadecyl phase provides hydrophobic retention, while extensive endcapping minimizes secondary interactions at residual silica sites. Larger packing particles can affect efficiency, permeability and operating pressure compared with finer particle variants, so established methods require an appropriate performance assessment before substitution. The long column should be equilibrated thoroughly before measuring retention and resolution. Select compatible mobile phases, avoid insoluble sample material and inspect fittings for leaks. Backpressure and observed plate count depend on solvent composition, temperature, flow and system connections. This product is one complete analytical column; it is not a preparative bulk packing material or a replacement guard cartridge.
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