Description
Product Overview Bone marrow mononuclear cells (BMNC) are a mixed population of single nucleus cells including monocytes, lymphocytes, and hematopoietic stem and progenitor cells. Cryopreserved Lonza Bone Marrow Mononuclear Cells are isolated from whole bone marrow via density gradient separation. Isolating mononuclear cells eliminates erythrocytes and graulocytes, producing a more stable cell product. Lonza BMNCs are available fresh* or frozen from a large and varied donor pool. They are guaranteed to contain either ≥5, 25, 100, 200 and 300 million viable cells per ampoule, depending upon product purchased. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Choices in Cell Type and Tissue Source Lonza offers choices in cell type and tissue source so you can choose the right product for your research and budget needs. Depending upon your ultimate cell of choice, you can choose from unprocessed bone marrow, bone marrow MNCs, bone marrow CD34+ cells, bone marrow stromal cells (for use as feeder cells), or bone marrow mesenchymal stem cells. In addition, if bone marrow is not your preferred tissue source, we also offer cord blood derived cells. Recommended Media and Reagents HPGM TM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application). Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations. Lonza guarantees the performance of Clonetics TM /Poietics TM cells only if appropriate Clonetics TM /Poietics TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. *Fresh cells are suspended in HBSS with 5 mM EDTA and 0.5% BSA and are available in a variety of cells per vial packaging sizes. Please contact Customer Service for information on sizes and pricing for fresh cells. Request More Info
Benefits Test negative for mycoplasma, bacteria, yeast, and fungi HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots A Certificate ofAnalysis is provided for each cell lot purchased
Storage and Content 1 x Cryopreserved ampule BMNCs containing ≥5 million cells
