Description
Product Overview CD19+ B cells are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using hemacytometer (trypan blue method). Cells are collected from healthy donors following IRB protocols. Request More Info
Applications Generating antibody libraries Co-culture with T helper cells or cytotoxic lymphocytes (CTL) B cell activation, clonal expansion, Ig rearrangement, and Ig secretion Antigen presenting cell (APC) signal transduction and antigen presentation
Storage and Content 1 vial containing ≥ 10 million cryopreserved cells