Lonza, BE02-059Q, Ferric Citrate Agent for Anti-clumping, 1 L Bottle

Name: Ferric Citrate Agent for Anti-clumping, 1 L Bottle
Brand: Lonza
SKU: BE02-059Q

Size: 1 L, Ferric Citrate (100x) Agent, 1 L (100x)

Catalog Number: BE02-059Q

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Category

Trace Elements

Pack Size1 L

TagsCulture Media and Reagents

Description

Product Overview Chinese Hamster Ovary (CHO) cells are widely used in large-scale culture. These cells have conventionally been grown as attached cultures, but they will also grow in suspension, a property which has allowed for relatively simple scale up in stirred tank and airlift bioreactors. Unfortunately, some CHO-derived lines which have been adapted to suspension growth can form large, tightly bound aggregates in culture. Why is clumping a problem? In batch suspension cultures, cell aggregation hinders accurate cell counting, monitoring and control of the cellular environment Transport of nutrients to, and products from, the cells may be impaired Cluster formation dramatically influences the growth behavior of the cells Cells within aggregates show a strongly reduced proliferation rate Shear forces exerted on large aggregates cause a considerably higher specific death rate than those exerted on single cells, reducing the specific growth rate up to 50 % To solve the problem of cell aggregation, Lonza introduces Anti-clumping Agents A (BE02-058E) and B (BE02-059E, F and Q-Ferric Citrate Agent for anti-clumping), two ready-to-use solutions designed to minimize CHO cell clumping in suspension cultures. Both solutions are made of non-animal origin components. Anti-clumping Agent A is a buffered, concentrated solution of Dextran sulfate, and recommended usage is at 100 × dilution Anti-clumping Agent B is a buffered, concentrated solution of Ferric Citrate Agent for anti-clumping, and recommend use it at 100 × or 200 × dilution As a result of using anti-clumping agents from Lonza, clumping of cells cultured in serum-free medium is minimized, leading to a significant increase in cell density and productivity. This BioWhittaker® Cell Culture Media is produced according to cGMP and labelled "For Further Manufacturing" (FFM). GMP Products are intended for research or further manufacturing use only. THIS PRODUCT IS NOT INTENDED FOR DIRECT THERAPEUTIC USE IN HUMANS. If you require different pack sizes, contact Lonza for more details. Request More Info
Benefits Anticlumping agent to maximize cell growth Easily scaleable to support high-density, large scale production volumes Ferric citrate agent for anti-clumping Applications High yield protein production in CHO cells Included in powder kit PowerCHO® 2 and Feeds
Storage and Content 1 L bottle

FAQs

Question: What type of cell culture is this supplement intended to support?

Answer:It is intended for anti-clumping use in suspension cultures, including CHO-derived cell lines that form aggregates.

Question: What concentration format is supplied?

Answer:The product is supplied as a 100X ferric citrate agent.

Question: What bottle size is supplied?

Answer:This configuration contains 1 L.

Application Note

Ferric Citrate Agent for Anti-clumping, 100X, 1 L is used as a concentrated cell-culture supplement to help manage aggregation in suspension-adapted Chinese hamster ovary cell cultures. Some CHO-derived production lines can form large, tightly bound cell aggregates after adaptation to suspension growth, which can complicate mixing, sampling, cell counting, and scale-up. This 100X ferric citrate agent provides a defined supplement format for anti-clumping workflows in large-scale CHO culture. The 1 L bottle supports repeated preparation of supplemented culture medium and is suited to bioreactor-oriented processes where consistent suspension behavior is important for monitoring and process control. Because the product is supplied as a 100X concentrate, it can be incorporated into established medium-preparation procedures without adding large liquid volumes. The product is intended for research and bioprocess cell-culture use where aggregation control is part of maintaining a more uniform suspension culture.

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