Lonza, LT07-610, PPiLight® Inorganic Pyrophosphate Assay, 500 Test

Product Overview Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3), ligases (EC 6) and DNA polymerases such as DNA Polymerase theta (θ) encoded by POLQ. The PPiLight® Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin. Request More Info
Benefits Fast-Measure enzyme activity via pyrophosphate consumption in 1 hour Simple-The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substrates Wide Detection Range-Sensitive to 0.02 µM with a linear range to 10 µM Versatile-Scalable to 96-, 384-, 1586-well formats Applications Measure activity of: Phosphotransferases Pyrophosphotases Cyclases Ligases Hydrolases DNA Polymerases
Storage and Content 1 x LT27-263 PPiLight® converting reagent, 10 mL 1 x LT27-260 PPiLight® detection reagent, 10 mL 1 x LT27-266 PPiLight® reconstitution buffer, 20 mL Storage Conditions: 2°C-8°C