Description
Product Overview The P3 Primary Cell 4D-Nucleofector TM LV Kit L is suited for larger scale transfection of primary cells in the 1 mL Nucleocuvette TM Cartridge format when working with the 4D-Nucleofector TM LV Unit. With the 1 mL Nucleocuvette TM Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port. This kit has been determined to be optimal for transfecting following primary cells and many others that use Solution P3 as optimal Nucleofector TM Solution: B cell (human) CD34+ cell (human) DC (human) ES (human), H9 Macrophage (human) Monocyte (human) T cell (human), stim. T cell (mouse), unstim. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. *Note: From the five primary cell solutions (P1 – P5) that come with the 4D-Nucleofector TM System only P3 is available for the large-scale formats so far. Solution P3 is suited for most immune and stem cells (e.g. T cells, dendritic cells, CD34 hematopoietic stem cells). For other solutions please contact our Scientific Support. Request More Info
Benefits Closed Nucleofection of up to 10 8 primary cells Conditions transferable from existing smaller scale protocols Simple manual filling via sterile injection port Transfection within seconds Applications For large scale transfection of up to 1 x 10 8 cells Ex-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells) Generation of large numbers of transiently modified primary cells for cell-based assays
Storage and Content 2.25 mL P3 Primary Cell Nucleofector TM Solution 0.5 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 1 mL Nucleocuvette TM Cartridge
