Product Overview The SF Cell Line 96-well Nucleofector® Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector® 96-well Unit. This kit has been determined to be optimal for transfecting following cell types: 293-F (FreeStyle), 32D A-20, A2058, A375, A431, A549, AGS, ARPE-19 BetaTC/6, BxPC-3 C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1 EL4 FDC-P1 H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C IMR-32 J774A.1 K-562 KG-1 L-428, LNCaP MDA-MB-453, MDBK, Molt-4, MV-4-11 Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0 P19, P815, PC-12, PC-3 Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226 S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480 T2, T84, TF-1 U266B1, U-937 Vero The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. The kit is available in different variants for either 1 x 96 or 10 x 96 reactions. Request More Info
Benefits High transfection efficiencies in difficult-to-transfect cell lines Compatible with liquid handling systems for automated high-throughput electroporation Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System Applications Transfection of low cell numbers in the range of 5 x 10 4 to 1 × 10 6 cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content 2.25 mL SF Cell Line Nucleofector® Solution 0.5 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 1 x 96-well Nucleocuvette® Plate (20 μL)
