Description
Product Overview The Primary Cell Optimization Nucleofector® Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit. With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well Nucleocuvette® Plate with different programs, primary cell optimizations are easily performed within one experiment. Complete Optimization: Using one 96-well Nucleocuvette® Plate and our pmaxGFP TM Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette® Plate can be used for optional fine tuning or performing the first experiment with the selected conditions. Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs. Basic Protocols are available for following cell groups: Endothelial cells (mammalian) Epithelial cells (mammalian) Fibroblasts (mammalian) Glial cells (mammalian) Neurons (mammalian) Smooth muscle cells (mammalian) Stem cells (human) Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data. Request More Info
Benefits Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well Nucleofector® System Applications Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit
Storage and Content 0.675 mL Nucleofector® Solution P1 0.675 mL Nucleofector® Solution P2 0.675 mL Nucleofector® Solution P3 0.675 mL Nucleofector® Solution P4 0.675 mL Nucleofector® Solution P5 4 x 0.15 mL Supplement 1 1 x 0.15 mL Supplement 3 2 x 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 96-well Nucleocuvette® Plates
