Product Overview The SF Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit. This kit has been determined to be optimal for transfecting following cell types: 293-F (FreeStyle), 32D A-20 , A2058, A375 , A431 , A549 , AGS , ARPE-19 BetaTC/6, BxPC-3 C6 , CCRF-CEM , CHO-K1 , CHO-S (Invitrogen) , Cos-1 EL4 FDC-P1 H9c2 (2-1) , HEK293 , Hel9217 , HepG2 , High 5, HL-60 , HMC-1-8 , HT-1080 , HT-29 , Hut-78 , HUV-EC-C IMR-32 J774A.1 K-562 KG-1 L-428 , LNCaP MDA-MB-453 , MDBK , Molt-4 , MV-4-11 Nalm-6 , NB-4, NCI-H1299 [H1299] , Neuro-2a [N2a] , NG108-15, NS0 P19 , P815, PC-12 , PC-3 Raw 264.7 , RBL-1, RBL-2H3 , Reh, Rin m5F , RPMI 8226 S2, S49 , SaOS , SF9, SH-SY5Y , SK-BR-3 , SK-N-AS , SK-N-MC , SK-N-SH , SK-OV-3 , Sp2/0-Ag14 , SW480 T2, T84, TF-1 U266B1, U-937 Vero The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. Request More Info
Benefits High transfection efficiencies in difficult-to-transfect cell lines Cells maintain functionality post transfection Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System Transferability to 4D-Nucleofector® LV Unit Applications Transfection of low cell numbers in the range of 5 x 10 4 to 1 x 10 6 cells Medium throughput applications Optimization of transfection conditions e.g. substrate titrations
Storage and Content 1 x 0.675 mL SF Cell Line Nucleofector® Solution 1 x 0.15 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 16-well Nucleocuvette® Strips (20 μL)
