Description
Product Overview The SG Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit. This kit has been determined to be optimal for transfecting following cell types: Ba/F3 Colo-205, CHO-S H4 NIH/3T3 Raji , Ramos THP-1 WEHI The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection® conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. Request More Info
Benefits High transfection efficiencies in difficult-to-transfect cell lines Cells maintain functionality post transfection Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System Applications Transfection of low cell numbers in the range of 5 x 10 4 to 1 x 10 6 cells Medium throughput applications Optimization of transfection conditions, e.g. substrate titrations
Storage and Content 1 x 0.675 mL SG Cell Line Nucleofector® Solution 1 x 0.15 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 16-well Nucleocuvette® Strips (20 μl)