Description
Product Overview The P3 Primary Cell 384-well Nucleofector® Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT Nucleofector® System. This kit has been determined to be optimal for transfecting following cell types: B cell (human) CD34+ cell (human) Chondrocyte (human) DC (human) DC (mouse), mature ES (human), H9 ES (mouse) Hepatocyte (human) HMEC (human) Keratinocyte (human) Macrophage (human) Monocyte (human) Neuron (rat), brain Hi/Cx NHBE (human) RPE (human) T cell (human), stim. T cell (mouse), unstim. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge databas e . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. The kit is available in different variants for either 2 x 384 or 10 x 384 reactions. Request More Info
Benefits High transfection efficiencies primary cells-Cells maintain functionality post transfection Seamless integration into automated liquid handling environments Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System Applications Transfection of low cell numbers down to 2 x 10 4 primary cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content 90 mL P3 Primary Cell Nucleofector® Solution, 20 mL Supplement 1, 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0), 10 384-well Nucleocuvette® Plate (20 μl)
