Description
Product Overview The Cell Line Nucleofector® Kit V is one of our five Nucleofector® Kits suited for transfecting cell lines in the Nucleofector® 2b System. This kit has been determined to be optimal for transfecting following cell types: 32D 3T3-L1 pre-ad A20 A375 A7r5 AGS ARPE-19 B16-F10 BA/F3 (DSMZ) C2C12 C6 Capan-1 CHO [suspension] CHO-S [suspension] (Life Technologies) COS-1 HaCaT HCT116 HEK293 HepG2 HL-60 HUV-EC-C Jurkat K-562 (DSMZ) MCF7 MDA-MB-231 MDA-MB-468 NB-4 (DSMZ) NCTC clone 929 Neuro-2a [N2a] NG108-15 P19 PC-12 PC-3 Raji Ramos RAW 264.7 RBL-1 S49 Saos-2 Schneider‘s Drosophila Line 2 SH-SY5Y SK-N-SH SK-OV-3 SW480 T/C-28 a2 T/G HA-VSMC T-47D THP-1 U-2 OS Vero The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database . In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type. Request More Info
Benefits High transfection efficiencies in difficult-to-transfect cell lines Applications Transfection of cell numbers in the range of 2 × 10 5 to 2 × 10 7 cells Low-throughput transfection in single cuvette format
Storage and Content 2.25 mL Nucleofector® Solution V 0.5 mL Supplement 1 30 µg pmaxGFP TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 25 Aluminum cuvette (100 µL) 25 Single use pipettes
