Description
Product Overview The Cell Line Nucleofector® Kit C is one of our five Nucleofector® Kits suited for transfecting cell lines in the Nucleofector® 2b System. This kit has been determined to be optimal for transfecting following cell types: A2058 CCRF-CEM MDA-MB-453 MEG-01 MG-63 NCI-H1299 [H1299] NS0 (ECACC) SK-BR-3 T2 U266B1 U-937 The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database . In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type. Request More Info
Benefits High transfection efficiencies in difficult-to-transfect cell lines Applications Transfection of cell numbers in the range of 2 × 10 5 to 2 × 10 7 cells Low-throughput transfection in single cuvette format
Storage and Content 2.25 mL Nucleofector® Solution C 0.5 mL Supplement 2 30 µg pmaxGFP TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 25 Aluminum cuvette (100 µL) 25 Single use pipettes