Merck, 08445-100MG, 7-Amino-4-methyl-3-coumarinylacetic acid

Application
AMCA is a useful agent for labeling proteins. Demonstrates a characteristic absorption peak at 345 nm with a fluorescence emission at 440-460 nm (blue region); however, when conjugated with proteins the absorption peak shifts to 355 nm with a fluorescence emission at 440-460 nm. It has a high fluorescence yield with a sufficient Stokes shift (100 nm) and is relatively photostable . Relatively easy to activate and couple to the N-terminal amino group of a peptide resulting in an acid stable amide bond .
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Useful blue fluorophore for immunofluorescence, λabs ~350 nm, λem ~440 nm