Merck, 10109878001, Trypsin Inhibitor

Name: Trypsin Inhibitor
Brand: Merck
SKU: 10109878001

Size:1 g, trypisin, from chicken egg white

Catalog Number: 10109878001

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Category

Trypsin Solutions

TagsCell Culture & Analysis Cell Culture Supplements & Reagents Cell Dissociation Reagents

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).
Application
Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:

as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
to neutralize trypsin post dissociation and trypsinization of brain tissues
termination of tissue disaggregation and for subcultivation procedures

Preparation Note
Working concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.
Store at 2 to 8 °C. (Store dry!)
Other Notes
For life science research only. Not for use in diagnostic procedures.

Specifications

General

UNSPSC Code	12352207
NACRES	NA.54
biological source	chicken egg white
form	powder
packaging	pkg of 1 g
manufacturer/tradename	Roche
concentration	1 mg/mL
technique(s)	cell culture \| mammalian: suitable
optimum pH	3.8-4.5
solubility	water: soluble
storage temp.	2-8°C

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