Merck, 10109886001, Trypsin Inhibitor

General description
Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.
Application
Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
in the buffer for hepatocyte isolation
to inhibit trypsin in Corneal epithelial cells
to inhibit papain activity while treating hippocampal neural stem cells (NSCs)

Biochem/physiol Actions
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.
Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Features and Benefits
Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)

Isoelectric point: 4.5
Preparation Note
Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Sensitive to heat, alkaline pH, and protein-precipitating compounds.
Store at 2 to 8 °C. (Store dry!)
Other Notes
1 U = 270 S & T-units or 90 USP-units
For life science research only. Not for use in diagnostic procedures.