Merck, 1055620001, TLC plates, Silica gel 60 F₂₅₄

General description
1 Aluminium roll 500 x 20 cm
Our classical silica TLC plates are based on a combination of our proven silica gel 60 and the addition of a unique polymeric binder resulting in a very adherent and hard surface that will not crack or blister and even allow writing with a pensil on the surface without risk to damage the layer.
Application

Determination of β-blocking receptor drugs in silica gel TLC systems with the mobile phase containing surfactant.: This paper investigates the use of silica gel TLC plates with surfactant-containing mobile phases for the separation and quantitative analysis of β-blocking receptor drugs. The environmentally friendly method provides accurate LOD and LOQ values for various drugs (Traczuk and Polak, 2022).

Separation of peptides in thin-layer chromatography systems with C18 silica-based adsorbents.: This paper investigates the separation of peptides using thin-layer chromatography (TLC) systems with C18 silica-based adsorbents, including Silica gel 60 F254 plates. The study demonstrates the utility of these systems in peptide analysis (Gwarda et al., 2016).

Analysis Note
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.7 - 11.7 µm
Layer thickness: 160 - 210 µm
Deviation of layer thickness per plate: ≤ 40 µm
Chromatographic testing:
Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
11 - 25
- Ceres Black G, colour test, lipophile: 34 - 48
- ceres Violet brn, colour test, lipophile: 52 - 67
Typical value determined on a conditioned sheet
Eluent: toluene (45% rel. humidity)
Other Notes
Replaces: 5562-7; 5562