Merck, 11685678001, Agarose LE

Name: Agarose LE
Brand: Merck
SKU: 11685678001

Size:500 g, agarose, low electroendosmosis

Catalog Number: 11685678001

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Category

Agarose

Pack Size500 g

TagsAgarose Chemistry & Biochemicals Biochemicals

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.
Application
Agarose LE has been used for:

analyzing fragments between 0.2 and 15 kb
analysis of PCR products
examination of restriction endonucleases
digests of plasmid, cosmid, and λ phage DNA
electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
in combination with ethidium bromide in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.
Analysis Note
Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.
Other Notes
For life science research only. Not for use in diagnostic procedures.

Specifications

General

UNSPSC Code	12352200
NACRES	NA.54
biological source	algae (seaweed)
form	powder
packaging	pkg of 100 g (11685660001)
manufacturer/tradename	Roche
EEO	0.05 -0.13
transition temp	gel point 36 °C ±1.5
gel strength	>=2,500 g/cm2
foreign activity	Dnase, none detected
shipped in	ambient
storage temp.	20-25°C

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