Merck, 15024AST, Astec® CHIROBIOTIC® V2 Chiral HPLC Column

Name: Astec® CHIROBIOTIC® V2 Chiral HPLC Column
Brand: Merck
SKU: 15024AST

Size:1 ea, Chirobiotic V2 TM=["Astec"] Chromatography Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Catalog Number: 15024AST

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Category

Chiral HPLC Columns

Pack Size1 ea

TagsHPLC Columns Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC® V in the polar ionic mode. The CHIROBIOTIC® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

Bonded phase: Vancomycin
Operating pH range: 3.5 - 7.0
Particle diameter: 5, 10 or 16 μm
Pore size: 100 Å (CHIROBIOTIC® V) or 200 Å (CHIROBIOTIC® V2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature
Application

Chiral analysis of dextromethorphan and levomethorphan in human hair by liquid chromatography-tandem mass spectrometry: This study utilized the CHIROBIOTIC® V2 column to achieve chiral separation of dextromethorphan and levomethorphan in human hair, highlighting the column′s application in forensic toxicology for precise enantiomeric analysis (Ji et al., 2022).

Development and validation of an UFLC-MS/MS method for enantioselectivity determination of d,l-thero-methylphenidate, d,l-thero-ethylphenidate and d,l-thero-ritalinic acid in rat plasma: This research demonstrates the application of CHIROBIOTIC® V2 for pharmacokinetic studies in rats, establishing a method for enantioselective determination that can be crucial for drug development and therapeutic monitoring (Zhang et al., 2016).

New high-performance liquid chromatography method for the determination of (R)-warfarin and (S)-warfarin using chiral separation on a glycopeptide-based stationary phase: The use of CHIROBIOTIC® V2 column in developing a new HPLC method for the separation of warfarin enantiomers underscores its utility in clinical analysis and therapeutic drug monitoring, showing its broad applicability in biomedical research (Malakova et al., 2009).

Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Specifications

General

UNSPSC Code	41115700
eCl@ss	32110501
Web Name Suffix Override	L × I.D. 25 cm × 4.6 mm, HPLC Column
Web Name Override	Astec® CHIROBIOTIC® V2 Chiral (5 μm) HPLC Columns
NACRES	SB.52
material	stainless steel column
agency	suitable for USP L88
description	HPLC column
product line	Astec®
packaging	pkg of 1 ea
manufacturer/tradename	Astec®
parameter	0-45 °C temperature
technique(s)	HPLC: suitable
L × I.D.	25 cm × 4.6 mm
matrix	High-purity silica gel particle platform
matrix active group	vancomycin phase
particle size	5 μm
pore size	200 Å
operating pH range	3.5-7.0
separation technique	chiral
Quality Level	100

FAQs

Question: What is the stationary phase?

Answer:Vancomycin-derived CHIROBIOTIC V2 chiral phase on porous silica.

Question: What are the dimensions and particle size?

Answer:250 mm length × 4.6 mm internal diameter with 5 µm particles and 200 Å pores.

Question: What pH and pressure limits apply?

Answer:Nominal pH 3.5–7.0 and maximum 241 bar (3,500 psi).

Application Note

The Supelco Astec CHIROBIOTIC V2 Chiral HPLC Column uses a vancomycin-derived chiral stationary phase for separating enantiomers of suitable pharmaceutical and analytical compounds. The 250 × 4.6 mm stainless steel analytical column contains 5 µm fully porous high-purity silica particles with a nominal 200 Å pore size. Multiple recognition sites within the macrocyclic glycopeptide stationary phase allow enantioselective interactions involving hydrogen bonding, ionic and steric contributions under an appropriate mobile phase. This makes the phase useful for developing methods for amines, amides, acids, esters and other molecules whose stereoisomers require chromatographic resolution. The column supports suitable HPLC and LC-MS workflows and can be used with a range of mobile-phase modes, including compatible polar ionic conditions. Follow the defined solvent and buffer constraints; the nominal operating pH range is 3.5–7.0, with a temperature range of 0–45 °C and pressure limit of 241 bar (3,500 psi). Optimize salt and organic modifier composition for the analyte, and validate resolution and retention before routine testing. This is a single V2 analytical column, not the original CHIROBIOTIC V phase or a guard column.

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