Merck, 3055-100GM, CHAPS

Name: CHAPS
Brand: Merck
SKU: 3055-100GM

Size:100 g, CHAPS, 3-[(3-Cholamidopropyl)-dimethylammino]-propane- sulfonate, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, OmniPur® Grade, Zwitterionic detergent

Catalog Number: 3055-100GM

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Category

Zwitterionic Detergents

Pack Size100 g

TagsChemistry & Biochemicals Biochemicals Detergents

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Zwitterionic detergent ideal for solubilization of membrane proteins, analytical, diagnostic, chromatography, electrophoresis work, and enzymology and immunology applications.
Suitable for Molecular Biology

Conductivity (10%, water): ≤50 µmho
DNase: None detected
Protease: None detected
Purity (TLC): Single spot
RNase: None detected
Solubility: 20% in water, passes test

Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
Application
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

Web Name Suffix Override	OmniPur® Grade
NACRES	NA.25
UNSPSC Code	12161703
MDL number	MFCD00012116
InChI	1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
SMILES string	C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI key	UMCMPZBLKLEWAF-RFCNGIAKSA-N
form	crystalline powder
mol wt	micellar avg mol wt 6150
manufacturer/tradename	Calbiochem®
storage condition	OK to freeze
aggregation number	10
color	white
CMC	6 mM (20-25°C)
transition temp	cloud point >100 °C
foreign activity	DNAse, none detected
shipped in	ambient
storage temp.	10-30°C
Quality Level	200

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