Merck, 35845-1G, DCFHDA

Name: DCFHDA
Brand: Merck
SKU: 35845-1G

Size:1 g, 2′, 7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein, BioReagent, suitable for fluorescence, ≥95% (HPLC)

Available Catalog Numbers

35845-1G 35845-5G

Catalog Number: 35845-1G

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Category

Special Stains

Pack Size1 g

TagsClinical & Diagnostics Tissue Diagnostics Dry Dyes & Certified Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.
Application
2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.

Specifications

General

UNSPSC Code	12352108
NACRES	NA.32
PubChem Substance ID	57649438
MDL number	MFCD00037501
Beilstein/REAXYS Number	68488
InChI key	VQVUBYASAICPFU-UHFFFAOYSA-N
InChI	1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
SMILES string	CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
Quality Level	100
description	suitable as a fluorogenic substrate for esterases
product line	BioReagent
assay	≥95% (HPLC)
form	powder or crystals
mol wt	485.27 g/mol
color	faint red
mp	232-234 °C (lit.)
solubility	DMSO: soluble
fluorescence	λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
suitability	suitable for fluorescence
storage temp.	2-8°C

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