Description
General description
7α-Hydroxy-4-cholesten-3-one is an intermediate in the biochemical synthesis of bile acids from cholesterol. Its precursor, 7α-hydroxycholesterol, is produced from cholesterol by hepatic cholesterol 7α-hydroxylase (CYP7A1).[1]It is metabolized by the enzyme 7α-hydroxycholest-4-en-3-one 12α-hydroxylase to 7α,12α-dihydroxycholest-4-en-3-one and then to cholic acid, the major primary bile acid in humans. Alternatively, it can be converted into 5β-cholestane-3α,7α-diol and then to chenodeoxycholic acid, the other major primary bile acid in humans.Serum 7α-hydroxy-4-cholesten-3-one concentrations reflect the activity of the bile acid synthetic pathway. Serum 7α-hydroxy-4-cholesten-3-one values vary during the day as bile acid synthetic rates have a diurnal rhythm.[2]Elevated values are found in patients with bile acid malabsorption and may be useful in the diagnosis of this condition as high values are associated with low SeHCAT retention.[3] The increase in serum 7α-hydroxy-4-cholesten-3-one concentrations reflects the loss of bile acids secondary to bile acid malabsorption or the increased synthesis found in primary bile acid diarrhea associated with impaired negative feedback of CYP7A1 by FGF19.[4]
Biochem/physiol Actions
7α-Hydroxy-4-cholesten-3-one is metabolized by the enzyme 7α-hydroxycholest-4-en-3-one 12α-hydroxylase to 7α,12α-dihydroxycholest-4-en-3-one and then to cholic acid, the major primary bile acid in humans. Alternatively, it can be converted into 5β-cholestane-3α,7α-diol and then to chenodeoxycholic acid, the other major primary bile acid in humans. Serum 7α-hydroxy-4-cholesten-3-one concentrations reflect the activity of the bile acid synthetic pathway. Serum 7α-hydroxy-4-cholesten-3-one values vary during the day as bile acid synthetic rates have a diurnal rhythm. Elevated values of 7α-hydroxy-4-cholesten-3-one are found in patients with bile acid malabsorption. It a may be useful in the diagnosis of this condition as high values are associated with low 75-selenium homocholic acid taurine (SeHCAT) retention. The levels of 7α-hydroxy-4-cholesten-3-one is elevated in irritable bowel syndrome with diarrhea (IBS-D). Deuterium labeled 7α-hydroxy-4-cholesten-3-one (d7-7αC4) is employed as an internal standard in the liquid chromatography-tandem mass spectrometry (LC-MS/MS) measurements.
Packaging
5 mL Amber Glass Screw Cap Vial (700112P-10mg)
5 mL Amber Glass Screw Cap Vial (700112P-1mg)
5 mL Amber Glass Screw Cap Vial (700112P-5mg)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
