Merck, 83702-10MG, Rhodamine 123

General description
Rhodamine 123 is a fluorescent dye.
Application
Rhodamine 123 has been used:
as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
to label the cells for estimating the P-gp expression by flow cytometry
in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Other Notes
Supravital mitochondrial-specific dye