Merck, 860891-25G, Bromocresol Purple sodium salt

Name: Bromocresol Purple sodium salt
Brand: Merck
SKU: 860891-25G

Size:25 g, 5′, 5″-Dibromo-o-cresolsulfonphthalein sodium salt, indicator grade, Dye content 90 %

Available Catalog Numbers

860891-25G 860891-5G

Catalog Number: 860891-25G

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Category

Special Stains

Pack Size25 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Bromocresol Purple, also known as dibromo-o-cresolsulfonphthalein, is an acidic dye and belongs to the phthalein and sulphonphthalein dyes group. The dye color changes from yellow to violet at pH 5.2-6.8. The monosodium salt of the dye is 8% soluble in water and 2% in ethanol. Bromocresol Purple, under alkaline conditions, forms a hydrophilic, moderately sized dianion that is purple.
Application
Bromocresol Purple is widely used as a vital stain to trace fluid diffusion in the lymphatic system. In addition, the dye is suitable to differentiate dead or live yeast cells. As a pH indicator, Bromocresol Purple is used in microbial culture media, both broth, and agar. In analytical experiments, e.g., to stain carbonic anhydrase isoenzyme on cellulose acetate electrophoresis membranes, bromocresol purple is used.

Specifications

General

NACRES	NA.47
PubChem Substance ID	24888522
UNSPSC Code	12171500
EC Number	263-655-3
MDL number	MFCD00148896
InChI	1S/C21H16Br2O5S.Na/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21;/h3-10,24-25H,1-2H3;/q;+1/p-1
InChI key	WNOIORUCCYNQPE-UHFFFAOYSA-M
SMILES string	[Na+].Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c([O-])c(Br)c4
grade	indicator grade
form	powder
composition	Dye content, 90%
technique(s)	titration: suitable
color	brown to very dark brown, to Very Dark Red
visual transition interval	5.2-6.8, yellow to purple
mp	255 °C (dec.) (lit.)
λ<sub>max</sub>	379 nm
ε (extinction coefficient)	≥47000 at 587-591 nm in 0.1 M NaOH
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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