Merck, 94091320-1VL, 8D12

Immunogen
Guinea pig major basic protein
Application
Bioassay
Biochem/physiol Actions
8D12 and 8A12 used in a sandwich ELISA giving a sensitive assay for biological samples. The antibody is directed against the guinea pig major basic protein found in guinea pig eosinophils. It can be paired in a sandwich ELISA with 8A12.
Mouse Sp2/0 x Mouse spleen
Preparation Note
IDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 1-2x100,000 cells/ml; 5% CO₂; 37°C. Saturation density is reached at 1x1000,000 cells/ml.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].